摘要
目前动物克隆技术的应用因低效和后代生长发育异常等缺陷而受到限制。问题的根源在于对克隆基础的分子机理缺乏了解。为更好地了解该领域当前的进展 ,我们研读了相关的文献 ,包括核移植过程中核质互作、核重编程、线粒体命运、端粒变化以及X染色体失活等机理方面的著述。看来 。
Application of animal cloning is limited with especially low efficiency and birth defect cloned offspring.This is because molecular mechanism underlying animal cloning is not well known.For better understanding of current progress in this field,we gathered reports on molecular ways affecting the success of animal cloning,such as nuclear cytoplasm interaction,epigenetic reprogramming,mitochondrial fate,telomere changing and X chromosome inactivation,etc.It looks as if the application of some new techniques such as bio CMOS chip would help resolving the problems.
基金
科技部攀登专项 (95 -专 -0 8)
中国科学院知识创新工程重大项目 (KSCX1-0 5 -0 1)
国家自然科学基金重点项目(3 983 0 2 80 )
关键词
动物克隆
研究进展
分子机理
核重编程
重构胚
Animal cloning
Molecular mechanism
Nuclear reprogramming
Reconstructed embryo
