端粒酶hTERT基因在男性不育症病人睾丸组织中的表达及其意义

Expression of Telomerase Gene hTERT in Testes of Infertile Male and Its Significance

目的 :研究端粒酶hTERT基因在男性不育症病人睾丸组织中的表达及其意义。 方法 :应用核酸原位杂交技术检测 4 7例男性不育症病人和 10例正常睾丸组织中端粒酶hTERT基因的表达和定位 ,并应用HPIAS 10 0 0高清晰度图像处理系统对hTERT阳性信号进行图像分析。 结果 :端粒酶hTERT基因阳性表达与生殖细胞 (精原细胞、精母细胞、精子细胞 )的分布定位一致 ,并且其表达强度与男性不育症病人睾丸组织中的生殖细胞的数量和密度显著相关 ,在唯Sertoli细胞综合征病人睾丸组织中无端粒酶hTERT基因的表达。 结论

Objectives: To study the expression and the significance of telomerase gene hTERT in testes of infertile male. Methods: By using in situ hybridization(ISH) techniques,the expression of telomerase gene hTERT mRNA in testes of 47 infertile male and 10 normal testicular tissues were observed. Results: In male testes, there was a positive correlation between the expression of hTERT and the quantity and density of germ cells(spermatogonia, spermatocyte, spermatid). The expression of hTERT in some germinal cell of maturation arrest patients were not significantly different with those of normal. Conclusions: Our results suggest that the deficiency of telomerase might be a factor for germinal cell maturation arrest and there might be some other etiological factors in these patients.Our study provides experimental groundwork for the gene therapy of male infertility. Natl J Androl,2003,9(1):16 19