代谢酶基因多态性与前列腺癌易感性的关系

Relationship Between Genetic Polymorphisms of Metabolizing Enzymes and Prostate Cancer

目的 :探讨CYP1A1、NAT2基因多态性与前列腺癌易感性的关系。 方法 :应用PCR RFLP、ASA和自动实时荧光定量分析技术 ,分析 5 8例前列腺癌病人和 112例健康对照者CYP1A1和NAT2基因 4个位点的多态性 ,比较前列腺癌病人与对照组间频率差异。 结果 :前列腺癌组Ile Val多态位点各等位基因和基因型频率与对照组比较差异有显著性 (P <0 .0 5 ) ,其中等位基因G和GG基因型使患前列腺癌的危险度分别提高了 1.5 9倍 (P <0 .0 5 )和 3.0 6倍 (P <0 .0 5 ) ;前列腺癌组MspI多态位点各等位基因和基因型频率与对照组比较差异无显著性 (P>0 .0 5 )。前列腺癌组NAT2慢乙酰化基因型频率与对照组比较差异无显著性 (P >0 .0 5 )。 结论 :CYP1A1Ile Val基因多态与前列腺癌的发生可能有关 。

Objectives: To study the possible relationship between CYP1A1, NAT2 genetic polymorphisms and the susceptibility of prostate cancer. Methods: Firty eight patients with prostate cancer and 112 healthy cases were selected as the control randomly. NAT2 and CYP1A1 gene polymorphisms were analysed with the methods of PCR RFLP, ASA and real time fluorescence Light Cycler. The difference of frequency between the patients and the controls was compared. Results: Among prostate cancer patients and their matched controls, the frequencies of alleles and genotypes were significantly different with Ile Val gene Polymorphisms ( P < 0.0 5 ), in which the frequency of the allele G and GG genotypes were significantly higher than those in their matched controls with an odds ratio of 1.59 and 3.06 ( P < 0.05 ), respectively; No significant differences of the frequencies of the MspI alleles and genotypes were found between the patients with prostate cancer and the matched controls( P > 0.05 ). No significant differences of NAT2 slow acetylator genotype frequency were found between the controls and prostate cancer patients ( P > 0.05 ). Conclusions: The CYP1A1 Ile Val gene polymorphisms might be associated with the occurrence of prostate cancer, while MspI gene polymorphisms and NAT2 slow acetylator genotype might not be associated with the occurrence of prostate cancer. Natl J Androl,2003,9(1):32 35