精原细胞移植的发展与现状

Advances in Spermatogonial Transplantation

精原细胞移植 (ST)的原理与其他器官移植类似 ,主要通过显微外科技术 ,将供体精原细胞注入受体 ,利用受体微环境使供体细胞分裂分化为精子。根据供体类型以及实验目的 ,将ST分类。其中 ,同种间细胞移植已经获得成功 ,而异种移植 (以人、马、牛、大鼠等为供体 ,免疫缺陷小鼠为受体 )、冰冻细胞移植均未成功 ,其原因可能与免疫排斥反应有关。ST有利于 :①精子发生过程的研究 ,②男性不育症的研究 。

Spermatogonial transplantation (ST) is a novel technique which mechanism is similar to other transplantations. The donor testicular cells transferred into recipient testes by the microinjection can initiate spermatogenesis and produce sperm in the recipient testis. The homograft has got success. The xenograft in which (man, horse, bull or rat, can be the donor; immunodeficiency mouse can be the recipient) and cryopreserved germ cells can not clonise in the recipient testes. The reason may be immunoreaction. ST technique will be propitious to these fields, ①to investigate fundamental aspects of spermatogenesis; ②to regenerate spermatogenesis in infertile individuals; ③to develop transgenic animals. Natl J Androl,2003,9(1):58 60