p53、K-ras基因对大肠癌血管生成的调控作用

Study of angiogenesis in human colorectal carcinoma and its modulation by p53 and K-ras gene

目的 探讨p53、K ras基因对大肠癌血管生成的调控作用。方法 用PCR 单链构像多态性分析 (SSCP)和免疫组织化学的方法研究 68例大肠癌和癌旁组织以及 2 0例正常组织p53、K ras基因突变及血管内皮生长因子 (VEGF)和微血管密度的表达情况。结果 大肠癌组中p53、K ras基因突变率及VEGF的表达明显高于癌旁组 ,2组的阳性率分别为 47 1 % ,44 1 % ,55 9% (32 /68,30 /68,38/68) ;1 3 2 % ,7 4% ,1 1 8% (9/68,5/68,8/68)。 2 0例正常组织中未检出p53、K ras基因突变及VEGF的阳性表达。VEGF的表达与大肠癌的血管生成和转移显著相关 (r =0 82 0 ,P <0 0 1 )。p53、K ras均与VEGF表达显著相关 (P <0 0 1 )。结论 p53、K ras基因可上调VEGF的表达 ,p53、K

Objective To investigate the angiogenesis in human colorectal carcinoma and its modulation by p53 and K ras gene Methods The positive rate of p53 and K ras gene mutation, the expression of vascular endothelial growth factor (VEGF) and microvessel density in 68 cancer tissue, peritumoral tissue samples and 20 normal controls were studied by PCR SSCP and immunohistochemical methods Results The positive rate of p53 and K ras gene mutation and the expression of VEGF in cancer tissue(47 1%,32/68;44 1%,30/68;55 9%,38/68)were significantly higher than in peritumoral tissue (13 2%,9/68;7 4%,5/68;11 8%,8/68) p53, K ras gene mutation and the expression of VEGF were not detected in 20 normal tissue The expression of VEGF was closely related with the angiogenesis and metastasis of colorectal carcinoma( r =0 820, P <0 01) VEGF expression correlated with both p53 and K ras gene mutation( P <0 01) Conclusions p53 and K ras gene upregulated the expression of VEGF p53 and K ras gene might play an important role in modulating tumor angiogenesis