束缚应激致大鼠心肌损伤的线粒体膜机制探讨

Study on Mitochondrial Membrane Mechanism of Rat Heart Injury Induced by Constrainting Stress.

目的观察束缚应激对大鼠心肌细胞线粒体膜生物学特性和功能的影响 ,探讨应激性心肌损伤的线粒体膜机制。方法建立应激动物模型 ,分别束缚不同时间后处死所有大鼠 ,检测线粒体PTP开放程度、膜流动性的改变、膜脂质过氧化水平及线粒体呼吸功能变化。结果束缚应激导致线粒体膜PTP开放、膜流动性降低、膜脂质过氧化物生成增多及线粒体呼吸功能损害 ,且随着束缚时间增加 ,以上各项指标改变加剧。

Objective To investigate the effect of constrainting stress on biological characters and function of mitochondrial membrane in rat heart and to explore the possible mitochondrial membrane mechanism underlying stress induced heart injury. Method Stress animal model was established.After constrained for different times,all rats were killed and several indexes were examined. Result Constrainting stress can induce mitochondrial permeability transition,decrease of mitochondrial membrane fluidity,increase of the production of membrane lipid peroxidation and injury of mitochondrial respiratory function which is in time dependent manner.Conclusion Mitochondrial membrane impairment and its effects are the important mechanism of stress induced heart injury.