微波照射对小鼠海马细胞膜ATPase活性和离子通道的影响

Effects of Microwave Irradiation on ATPase Activity and Voltage Dependent Ion Channel of Rat Hippocampus Cell Membrane.

目的从电生理、酶学角度探讨微波照射对海马细胞的影响机理。方法用 2 4 5 0MHz连续波照射理疗机为照射源 ,以小白鼠为对象 ,观察微波照射强度为 1 0mW /cm2 时小鼠海马细胞膜Na+,K+ AT Pase、Ca2 +,Mg2 + ATPase活性、电压门控型Na+、K+、Ca2 +通道的变化情况 ,分别用组织化学染色法和膜片钳方法测定ATPase活性和离子通道功能。结果 1 )照射组海马Na+,K+ ATPase活性与对照组无显著差别 ,Ca2 +,Mg2 + ATPase活性比对照组显著降低 (P <0 .0 5 ) ;2 )照射组静息电位未发生显著变化 ,电压门控型Na+、K+、Ca2 +电流的诱导发生率显著地低于对照组 ,Na+电流峰值所在膜电位向去极化方向偏移 ,Na+电流衰减速率减慢 ,A电流的发生率显著低于对照。结论 2 4 5 0MHz微波照射小鼠 ,在 1 0mW /cm2 时 ,细胞的生存不会受影响 ,但海马细胞膜Ca2 +,Mg2 + ATPase活性受到抑制 ,电压门控型Na+、K+、Ca2 +离子通道受到损害 。

Objective To study the effect of microwave irradiation on hippocampus cell. Method Changes of ATPase activity and voltage dependent ion channel of hippocampus cell membrane were observed in mice exposed to 2 450 MHz microwave irradiation of 10 mW/cm 2 from a physical therapy machine. Histochemical method and patch clamp method were used to determine the activity of Na +,K + ATPase, Ca 2+ ,Mg 2+ ATPase and voltage dependent Na +,K +,Ca 2+ channels respectively. Result 1)Na +,K + ATPase activity of microwave irradiated mice showed no significant change as compared with the control, but the activity of Ca 2+ ,Mg 2+ ATPase decreased significantly(P<0.05);2)In microwave irradiated mice, Na +,K +,Ca 2+ current inducement rate in hippocampus neuron decreased significantly, the membrane voltage of Na + current peak shifted to depolarization,and the attenuation rate of Na + current and current A inducement rate decreased significantly as compared with control mice. Conclusion Irradiation of 2 450 MHz microwave at a doze of 10 mW/cm 2 was not fatal to mice hippocampus cell. But Ca 2+ ,Mg 2+ ATPase activity of hippocampal cell membrane and voltage dependent Na +,K +,Ca 2+ ion channel of hippocampal nervous were affected which would affect study and memory.