不同年龄双相情感性精神障碍与多巴胺D_2、儿茶酚氧位甲基转移酶基因多态性的关联分析

Association analysis of dopamine D_2 receptor and catechol-O-methyltransferase genes with bipolar affective disorder in different age groups

目的 探索多巴胺D2 受体 (DRD2 )基因及儿茶酚氧位甲基转移酶 (COMT)基因在双相情感性精神障碍 (BD)发病中的作用。方法 (1 )在临床主治医师诊断后 ,由研究者采用美国精神障碍诊断与统计手册第 3版修订本的定式检查提纲 (SCID P)对精神疾病患者进行面检 ,共选择符合标准的 1 0 5例BD患者 ,并将患者的发病年龄以≤ 2 5岁为早发组 ,>2 5岁为晚发组 ;(2 )对入组患者和对照组 (1 2 8名健康人 )按常规方法提取DNA ,然后对DRD2、COMT基因定型 ;(3)各组间构成比差异用 χ2检验进行统计学分析。结果DRD2、COMT基因多态性均与正常对照组间的差异无显著性 ,但发病年龄大于 2 5岁组与正常对照组之间DRD2基因多态性的差异有显著性 (χ2 =4 36 ,P =0 0 4 )。

Objective To explore the possible role of DRD2 and COMT genes in susceptibility to bipolar disorders (BD) Methods The subjects were 105 BD patients and 128 normal controls The diagnoses of BD were made according to the DSM Ⅲ R criteria for bipolar disorders based on the structured clinical interview schedule (SCID P) Using the age of 25 years at onset as the cut off, the patients were divided into early onset or late onset subgroup The genotypes of DRD2 and COMT genes were analyzed by the polymerase chain reaction based restriction fragment length polymorphism Results There were no significant differences of genotype or allele frequencies of the DRD2 and COMT genes between patients and controls But the polymorphisms of DRD2 gene were significantly different between controls and late onset patients Conclusion The results suggested DRD2 gene may be associated with late onset bipolar disorders