乙型肝炎病毒父婴传播途径的初步研究

Study on HBV transmission from father to infant

目的 探讨乙型肝炎病毒 (HBV)父婴传播中父亲与婴儿所携HBV基因型别的一致性 ,从而证明HBV父婴传播途径的存在。方法 选择父亲为HBV携带者而母亲无任何HBV感染标志的新生儿为研究对象 ,检测新生儿的HBV感染标志 ,比较父亲与子代所携HBVS基因 45 1～ 660段序列同源性并作基因分型。结果 16对父亲与婴儿所携HBV同属adw亚型 ,HBVS基因同源性在 98%～ 10 0 %之间 ,检出 488,491,494,5 3 0 ,5 3 1,5 46,5 81,62 1位核苷酸变异导致 112 ,113 ,114,12 6,13 1,143 ,15 6位氨基酸替代。以HBVS基因的分型方法 ,10对父婴所携HBV基因型别同属B型 ;4对同属A型 ;2对婴儿为A型 ,父亲介于A型与B型之间。

Objective To study HBV transmission from father to infant,the study was carried out.Methods The study contained 16 pairs of fathers who were HBV carriers,and infants whose mothers were not HBV carriers.The infants infected HBV in womb.The homogenous of HBS gene were compared between fathers and infants.Results The serotype of 16 pairs fathers and infants were adw.The homogenous of HBV S gene were 98%-100%.The mutation of 488,491,494,530,531,546,581 621nucleotide of S gene caused in 112,113,114,126,131,143,156 amino acid substitution.In the gene classification of HBV S gene,HBV of 10 pairs of fathers and infants were allocated group B.HBV of 4 pairs of fathers and infants were allocated to group A.Other 2 pairs,HBV of infants were allocated group A,while that of 2 fathers were allocated to group A and group B.Conclusion The HBV transmission from father to infant was existed.The HBV gene classification of fathers and infants was consistent.