不同免疫程序HFRS双价灭活疫苗免疫效果观察

Study on immune effect and persistence of bivalent inactivated vaccine with different immune schedule on epidemic hemorrhagic fever

目的 观察不同免疫程序肾综合征出血热 (HFRS)双价灭活疫苗的免疫效果 ,确定最佳免疫方案。方法 采用流行病学实验方法 ,将 15 8名 10～ 12岁儿童随机分组后按不同免疫程序进行免疫 ,基础免疫后 0 5个月、6个月、12个月、2 4个月及 2年加强免疫后 0 5个月采血检测HFRS荧光抗体及中和抗体。结果 2针 ( 0 ,14d)与 3针( 0 ,7,2 8d)组间、2针加强 ( 0 ,14d ,6个月 )与 3针加强 ( 0 ,7,2 8d ,6个月 )组间荧光抗体及中和抗体阳性率及GMT比无显著性差异 ;6个月加强 1针在免后 12～ 2 4月中 ,荧光抗体阳性率高于未加强组 ,短期 ( 2年内 )效果优于未加强组 ;免疫 2年加强免疫后 0 5个月 ,4组荧光抗体及中和抗体阳性率迅速升至 96 5 5 %～ 10 0 0 %。结论 既定 3针基础免疫程序可简化为 2针 ;

Objective To observe the immune effect of bivalent inactivated vaccine on epidemic hemorrhagic fever with different schedules,to determine the best immune schedules further.Methods To use the method of epidemiological experiment.According to the principle of random,158 children aged 10-12 and IFAT antibody negative were divided into 5 groups and allocated different immune process.The serum after being immunized two weeks and 0.5,6,12,24 month and 2 years were collected after a booster and indirect immunofluorescent method and Mcro-CEP method and the antibody in the serum was detected.Results The effect of bivalent inactivated vaccine on epidemic hemorrhagic fever with two doses in 0,14 days compared with three doses in 0,7,28 days existed no difference between them for two weeks to two years after being immunized.The effect of the vaccine with two doses in 0,14 days and a booster in 6 month compared with three doses in 0,7,28 days and a booster in 6 month existed no difference between them either.The effects to give a booster in 6 month were better than no booster in 1-2 years after being immunized.After the primary immunization for 2 years,a booster was given to all groups.The prevalence and GMT of antibody was increasing quickly.Conclusion The immune processo f three doses(0,7,28 dyas) may be simplified to become the immune process of two doses(0,14 days).It was better to give a booster in 6 month.