开心散有效部位对小鼠学习记忆功能的影响

Effects of KXS on Ability of Learning and Memory in Mice

用药理研究指导植化分离 ,寻找中药复方开心散有效部位 (KXS) ,探讨其益智作用及机理。采用氢溴酸东莨菪碱造模 ,用跳台法和水迷宫测试小鼠的学习记忆能力 ,测定小鼠大脑组织乙酰胆碱酯酶 (AchE)和超氧化物歧化酶 (SOD)活性以探讨其作用机理。跳台法测试表明模型组正确区时间 (Ts)缩短 ,KXS能延长Ts ,水迷宫实验结果显示 ,模型小鼠游泳时间明显延长 ,KXS能缩短游泳时间。KXS能逆转模型小鼠AchE活性的升高和SOD活性的降低。实验结果提示KXS对模型小鼠的学习记忆能力有一定的改善作用。

Objective: To study the effects and mechanisms of “kai xing san” (KXS) on ability of learning and memory in mice. Methods: Passive avoidance performance (Step-down test) and water maze methods were used to evaluate the effects of KXS on ability of learning and memory in mice. Spectrophotometry was employed to determine acetylcholine esterase(AchE) and SOD activities in whole brain. Results: In water maze, mice with memory impairments induced by Scopolamine hydrobromide took longer latency to reach the end point, while KXS can reduce the latency. In step down test, the right time (Ts) of the mice with memory disorders is shorter than that of the normal, while KXS can prolong Ts. KXS shows activities aganist the elevated AchE activity and the decreased SOD activity in whole brain of mice with memory disorders. Conclusion: KXS can improve the ability of learning and memory in models induced by Scopolamine Hydrobromide.