摘要
目的 :探讨参麦注射液对血管生成的影响 ,方法 :采用MTT法检测参麦注射液对牛血清和肿瘤细胞条件培养液促进的牛主动脉内皮细胞增殖的影响 ,采用琼脂糖刮除法检测参麦对牛血清和肿瘤条件培养液促进的牛内皮细胞迁移的影响。结果 :在含 10 %新生小牛血清培养液中和在肿瘤细胞条件培养液中 ,参麦都能明显抑制牛主动脉内皮细胞增殖 ,且呈量效关系 ,对肿瘤细胞条件培养液诱导的内皮细胞迁移抑制作用显著。结论 :参麦能抑制牛内皮细胞增殖和迁移 ,具有抑制血管生成的作用。
Object:To study the effects of Shenmai injection on angiogenesis. Methods:Proliferation of bovine aortic endothelial cells(BAECs) were measured by MTT colorimetric assay after treated by various concentrations of Shenmai injection. The effects of Shenmai injection on proliferation of BAECs induced by conditioned medium of tumor(CM) were also measured by the same method. The effect of various concentrations of Shenmai on the migration of BAECs induced by fetal bovine serurm(FBS) or CM was investigated by agarose assay. Results:Shenmai injection significanly inhibited proliferation of BAECs induced by FBS or CM. And Shenmai injection inhibited the proliferation of BAECs in a dose dependent manner. Shenmai injection inhibited migration of BAECs induced by FBS or CM. Conclusion:Shenmai injection can inhibit proliferation and migraion of bovine aortic endothelial cells. It is suggested that Shenmai can suppress angiogenesis.
出处
《中国实验方剂学杂志》
CAS
2003年第2期30-32,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
浙江省自然科学基金 (No .3 990 0 9)
浙江省中医管理局科研基金 (No .2 0 0 0C11)资助项目
