α,β,γ,δ-四(5-磺基噻吩)卟啉柱前衍生反相液相色谱法分离测定铜、镉和钴

Separation and determination of copper,cadmium and cobalt by reversed-phase liquid chromatography with α,β,γ,δ-tetrakis(5-sulfothienyl) porphyrin as the precolumn derivative agent

以α ,β ,γ ,δ 四 (5 磺基噻吩 )卟啉为柱前衍生试剂 ,用含 1 5mmol/LH3BO3-NaOH缓冲溶液 (pH9 0 )和 5 0× 10 - 3mol/L溴代三丁基胺 (TBA Br)的乙氰 -水 (3 0 :70 ,V/V)溶液作流动相 ,检测波长 43 0nm ,在KromasilC1 8柱上 ,同时分离测定了铜、镉和钴三种金属络合物 ,检出限 (S/N =3 )分别为 0 2 0 ,0 16,和 0 13ng/mL。方法用于水样分析 ,结果与光度法相符。

In this paper α,β,γ,δ-tetrakis(5-sulfothienyl) porphyrin is used as the precolumn derivative agent and the simultaneous determination of copper,cadmium and cobalt has been achieved in 13 min at 430nm on kromasil C 18 column with acetonitrile-water(30:70,V/V),contaning 1.5mmol/L H 3BO 3-NaOH(pH9.0) and 5.0×10 -3 mol/L TBA.Br as the mobile phase.The detection limits are 0.20,0.16 and 0.13ng/mL for copper,cadmium and cobalt respectively,when the ratio of signal to noise is 3.This method is sensitive and has been applied to the analysis of natural water sample.