一色齿毛菌对刚果红的脱色优化及其毒性变化研究

Optimization of decolorization and detoxification of Congo red by Cerrena unicolor

一色齿毛菌Cerrena unicolor是分离自野外的一株能够降解木质素的白腐真菌。为明确一色齿毛菌对染料的脱色能力及脱色前后染料毒性的变化,本研究利用一色齿毛菌对固体条件下4种染料进行脱色能力的检测,筛选出较易脱色的染料后,对该染料的脱色条件进行优化,并以3种豆类发芽率为指标测定该染料脱色前后的毒性变化。结果表明,一色齿毛菌对4种染料均可脱色,其中对刚果红的脱色效果最为明显;一色齿毛菌对刚果红脱色条件的优化结果为:20g/L麦芽糖,1g/L硝酸铵,1mmol/L硫酸镁,接种9块直径1cm菌饼,10mg/L染料浓度,pH7时脱色效果最好;刚果红染料脱色前后毒性测试结果显示:染料脱色前发酵液毒性>染料脱色后发酵液毒性>清水处理毒性,表明刚果红染料存在一定的毒性,但在被一色齿毛菌脱色后,染料毒性有所降低。本研究为一色齿毛菌在染料废水脱色方面的应用及降低染料废水毒性提供一定的参考依据。

Cerrena unicolor is a white rot fungus that can degrade lignin. In this study, the ability of C. unicolor to decolorize and detoxify four solid dyes was assessed. The decolorization conditions of the dyestuffs were optimized and the toxicity of the dyes was measured before and after decolorization. The results proved that C.unicolor could decolorize the four dyes, and the decolorization effect on Congo red was the most obvious. The optimized conditions for Congo red decolorization were: medium containing 20 g/L maltose, 1 g/L ammonium nitrate and 1 mmol/L magnesium sulfate, inoculated with nine pieces of fungal cake of 1 cm diameter size in10 mg/L Congo red solution adjusted at pH 7. The toxicity of Congo red before decolorization was greater than that after decolorization. It was evident that the toxicity of Congo red was reduced after degradation by C.unicolor. This study provides a reference for the application of C. unicolor in the decolorization and detoxification of dye wastewater.