深圳大鹏湾六种珊瑚共附生真菌多样性研究

Diversity of fungi associated with six species of coral in Dapeng Bay,Shenzhen, southern China

本研究使用6种不同培养基对深圳大鹏湾6种珊瑚上的共附生真菌进行平板涂布法分离培养,结合ITS-rDNA基因序列特征和形态学特征进行鉴定来研究深圳大鹏湾海域珊瑚共附生真菌的多样性。共分离培养出457株真菌菌株,分属于7个属,分别为红酵母属Rhodotorula(180株)、曲霉属Aspergillus(170株)、木霉属Trichoderma(50株)、青霉属Penicillium(34株)、枝孢属Cladosporium(21株)、透孢黑团壳属Massarina(1株)和弯颈霉属Tolypocladium(1株),其中优势属为红酵母属Rhodotorula和曲霉属Aspergillus,占菌株分离总数的76.59%。单独鹿角珊瑚上分离到的共附生真菌数量和种类最多,为160株9种;在该种珊瑚上还分离出弯颈霉属Tolypocladium sp.,该属真菌此前从未在珊瑚共附生真菌的研究中分离出来。本研究还发现SDA培养基分离出的共附生真菌数量最多,CDA培养基的真菌数量最少,表明不同培养基分离的共附生真菌多样性存在差异。

Six different kinds of culture media are used to isolate and cultivate the fungi associated with six species of coral collected from Dapeng Bay of Shenzhen.The identification was made through morphological observation in combination with ITS-rDNA blast homology analysis.A total of 457 fungal isolates were recovered and identified,belonging to seven genera including Rhodotorula(180 isolates),Aspergillus(170 isolates),Trichoderma(50 isolates),Penicillium(34 isolates),Cladosporium(21 isolates),Massarina(1 isolate),and Tolypocladium(1 isolate).Among them,Rhodotorula and Aspergillus were dominant,accounting for76.59%of the total number of isolates.The most species of fungi were isolated from Acropora solitaryensis,amounting to nine species(160 isolates).Tolypocladium sp.isolated from Acropora solitaryensis,has not been found before in corals.The most isolates were obtained from SDA medium,while only a few from CDA,indicating that the preference of fungi for culture media was different.