环链棒束孢Isaria cateinannulata发酵液的化学成分分析

Active components in extract from Isaria cateinannulata fermentative liquid

环链棒束孢广泛存在于自然界,并且在植物病虫害防治中起重要作用,目前相关的研究多集中于其发酵条件、生物学特性及杀虫毒力的研究,对该菌发酵液中的化学物质研究甚少。本文依次用石油醚和乙酸乙酯对环链棒束孢发酵液进行分级萃取,借助气相色谱-质谱(GC-MS)联用技术和超高效液相色谱串联四级杆飞行时间质谱法(UPLC/Q-TOF-MS)鉴定分析石油醚萃取物、乙酸乙酯萃取物和萃余物中的化学物质组成。共鉴定69个化合物的结构,其主要化学成分为酚类、酯类、烷烃、烯烃、卤代烃和酮类非极性/弱极性的小分子化合物等,其中邻苯二甲酸二丁酯、角鲨烯、环戊烯丙菊酯物质、瓜叶菊素Ⅰ等物质具有特殊的作用。发酵液提取物中还含有多种小分子化学物质,应结合多种色谱分离手段和波普学手段对代谢产物进行分离、纯化和结构鉴定,为其次生代谢产物的广泛利用提供数据支撑。

Isaria cateinannulata widely exists in nature and plays an important role in the control of plant disease and pest.At present,most of the related studies focus on the fermentation conditions,biological characteristics and insecticidal toxicity,while little research has been done on the chemical substances in the fungal fermentation broth.In this study,petroleum ether and ethyl acetate were used to extract the fermentation broth of Isaria cateinannulata.The chemical compositions of petroleum ether extract,ethyl acetate extract and raffinate of the fungus were identified and analyzed by gas chromatography-mass spectrometry(GC-MS)technology and high performance liquid chromatography tandem four-stage time-of-flight mass spectrometry(UPLC/Q-TOF-MS).A total of 69 compounds were identified,and the main chemical constituents were small molecular compounds such as phenols,esters,alkanes,olefins,halogenated hydrocarbons and ketones with non-polar/weak polarity.Among them,dibutyl phthalate,squalene,terallethrin and cinerinⅠhave special active effects.The fermentation broth extract also contained many kinds of other small molecular chemicals,and these metabolites yet await separation,purification and identification by using various chromatographic and spectroscopic methods.