柱状田头菇白色变种遗传分析

Genetic analyses of albino Agrocybe cylindracea

为分析柱状田头菇白色变种的遗传,以白色变种Ag.c0002和野生型Ag.c0067为亲本,通过原生质体单核化获得两亲本的单核菌株,配对杂交获得F1,从2个F1的子实体分离单孢菌株,与白色变种Ag.c0002的原生质体单核化菌株'2-2'进行回交配对,出菇观察子实体颜色,分析菇体颜色的遗传规律。结果显示,柱状田头菇子实体白色为隐性性状,且受单一位点基因控制,此外,菇体颜色基因与不亲和性因子A或B都没有连锁。

The genetic basis of albino Agrocybe cylindracea was investigated.Monokaryotic strains were obtained by means of protoplast monokaryolization of albino strain Ag.c0002 and brown isolate Ag.c0067.Monokaryotic strains from both parents were paired and four F1 hybrids were obtained.Single spore strains were isolated from fruit-body of two F1 and they were paired with monokaryotic strain'2-2'by back cross.F2 strains were cultivated and their fruit-body color was investigated for inheritance analysis.The results showed that fruit-body with white color had recessive character,and a recessive allele at a single locus encodes the white color.The allele did not link with incompatibility factor either A or B.