蝉棒束孢菌子实体对大鼠肾小管上皮细胞的影响

Effects of Isaria cicadae fruiting bodies on renal tubular epithelial cells in rats

对蝉棒束孢菌子实体(0.75g/kg)重复灌胃SD雄性大鼠90d及恢复28d的早期肾损伤生物标记物肾损伤分子-1(KIM-1)和中性粒细胞明胶酶相关载脂蛋白(NGAL)进行测定,评估蝉棒束孢菌子实体对肾小管上皮细胞的影响;研究不同剂量蝉棒束孢菌子实体(0.25g/kg、0.5g/kg、1.0g/kg)对肾小管上皮细胞增殖和增生能力的影响。给药30、60、90d及恢复28d时,SD大鼠血清中KIM-1浓度与对照组相比均无显著差异(P>0.05),给药30d、60d时,SD大鼠血清中NGAL浓度与对照组相比均无显著差异(P>0.05),给药90d及恢复28d时,SD大鼠血清中NGAL浓度低于对照组(P<0.05),且给药90d组与对照组相比有显著性差异(P<0.01);免疫组化检测增殖细胞核抗原法(PCNA)及四甲基偶氮唑盐微量酶反应比色法(MTT)表明:与对照组相比,蝉棒束孢菌子实体能使肾小管上皮细胞增生能力增强,未导致肾小管上皮细胞凋亡。

The effects of Isaria cicadae fruiting bodies(0.75 g/kg)on renal tubular epithelial cells was evaluated by measuring the biomarkers of kidney injury molecule-1(KIM-1)and neutrophil gelatinase-related apolipoprotein(NGAL)in SD male rats after repeated intragastric administration of Isaria cicadae fruiting bodies(0.75 g/kg)for 90 days and 28 days after recovery.The effects of different doses of Isaria cicadae fruiting bodies(0.25 g/kg,0.5 g/kg and 1.0 g/kg)on the proliferation and prolification of renal tubular epithelial cells were also studied.There was no significant difference in serum KIM-1 concentration between the experimental group and the control group in 30,60,90 days after administration and 28 days after recovery(P>0.05).In 30 and 60 days after administration,there was no significant difference in serum NGAL concentration between the experimental groups and the control group(P>0.05).In 90 days after administration and 28 days after recovery,the serum NGAL concentration of the experimental groups was lower than that of control group(P<0.05).Serum NGAL concentration in the experimental groups was lower than that in the control group in 90 days after administration and 28 days after recovery(P<0.05),and there was significant difference between the experimental group in 90 days after administration and the control group.Immunohistochemical detection of proliferating cell nuclear antigen(PCNA)and tetramethylazozolium salt microenzyme reaction colorimetry(MTT)showed that Isaria cicadae fruiting bodies could enhance the proliferation of renal tubular epithelial cells,but did not cause apoptosis of renal tubular epithelial cells.