烟管孔菌G11对活性染料的脱色

Decolorization of reactive dye by Bjerkandera adusta G11

本研究从未成熟的有机蓝莓表皮分离、纯化得到一株白腐真菌G11,通过对菌株G11的形态特征、ITS序列同源性比对以及系统发育分析,鉴定菌株G11为一株烟管孔菌Bjerkandera adusta。菌株G11可以产生木质素过氧化物酶、漆酶和锰过氧化物酶等木质素降解酶。菌株G11对8种不同染料的脱色效果显示其对活性染料的脱色效果最好,脱色率达到90%所需时间最短。以菌株G11为研究对象,研究其对不同浓度的活性黑和活性红的脱色能力,结果表明:菌株G11对活性红和活性黑具有显著的脱色能力。在脱色15d时,菌株G11对浓度为10、50、100、250、500mg/L活性红的脱色率分别为99%、98%、95%、94%和92%;对浓度为10、50、100、250、500mg/L活性黑的脱色率分别为98%、97%、95%、93%和90%。

A white rot fungus strain G11 was isolated and purified from the epidermis of immature organic blueberry.According to its morphological characteristics,ITS sequence homology and phylogenetic analysis,the strain was identified as Bjerkandera adusta.The fungus can produce lignin-degrading enzymes such as lignin peroxidase,laccase and manganese peroxidase.The decolorization assays to eight different dyes showed that it was the best of decolorization effect on reactive dyes,and it needed only a short duration to decolorize90%of reactive dyes.Further assay showed that the strain G11 had remarkable capability to decolorize reactive black and red,with decolorization rate of 99%,98%,95%,94%,and 92%respecfively for 10,50,100,250 and 500 mg/L reactive red,and 98%,97%,95%,93%,and 90%respectively for reactive black in decolorization duration of 15 d.