蝙蝠蛾拟青霉发酵滤液的成分组成与急性毒性分析

Composition and acute toxicity analyses of fermentation filtrate of Paecilomyces hepiali

蝙蝠蛾拟青霉是从新鲜的冬虫夏草上分离获得的虫草菌,可采用生物发酵制备大量菌丝体。该菌丝体具有和冬虫夏草相似的活性成分,具有抗肿瘤、抗病毒等多种功效。本研究对干燥后的蝙蝠蛾拟青霉发酵滤液的主要成分进行了分析,结果显示:干燥后的蝙蝠蛾拟青霉发酵滤液含粗脂肪21.33%,总蛋白27.34%,水解氨基酸16.79%,粗多糖11.16%,不饱和脂肪酸71.21%,其中以油酸、亚油酸为主;该干燥发酵滤液中还含有多种核苷类物质、多糖等生物活性成分以及多种人体必需的矿质元素。此外急性毒性实验的初步结果表明:小鼠在实验期内无死亡现象和明显的中毒反应,主要脏器的形态学正常;谷草转氨酶(AST)和谷丙转氨酶(ALT)活性与对照组相比无统计学差异。蝙蝠蛾拟青霉发酵滤液有望在医药和保健领域进一步研究开发。

Paecilomyces hepiali isolated from fresh Cordyceps sinensis stroma has active ingredients similar to those of Cordyceps sinensis,with anti-tumor,anti-virus and other effects.In this study,the main components of the dried fermentation filtrate of Paecilomyces hepiali were analyzed.The results showed that the dried fermentation filtrate of Paecilomyces hepiali contained 21.33%crude fat,27.34%total protein,16.79%hydrolyzed amino acid,11.16%crude polysaccharide and 71.21%unsaturated fatty acid including oleic acid and linoleic acid.The dried filtrate also contains a variety of nucleoside substances,polysaccharides and other biologically active ingredients,as well as essential trace elements.Preliminary results of acute toxicity experiments indicate that no death and obvious poisoning reaction occur in mice during experimental period,and the morphology of the main organs of mice was normal.There was no significant difference in the activity of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)as compared with the experimental control.Fermentation filtrate of Paecilomyces hepiali is worthy to be further researched and developed in the fields of medicine and health care.