体外消化对蓝莓提取物抗氧化、抗癌及组分的影响

Effects of in vitro Digestion on Antioxidant Activity, Anti-cancer Activity and Composition of Blueberry Extracts

以蓝莓为原料,考察了体外消化前、后蓝莓提取物(总酚和花色苷)含量、抗氧化性、抗癌作用及花色苷组成的变化,并推测了花色苷的降解途径。分别采用福林-酚法和pH示差法测定总酚和花色苷含量;以脂质体的抑制率、DPPH和ABTS+自由基清除能力评价体外消化前后提取物的抗氧化能力;利用高效液相色谱-电喷雾二级质谱联用技术分析花色苷组成。结果表明,与未经消化的提取物相比,经肠道消化后样品总酚含量增加47.21%,花色苷含量降低71.82%,脂质体的抑制率、DPPH和ABTS+自由基清除率分别提高38.45%、29.41%和29.12%;对Hep G2肝癌细胞、A549肺癌细胞和Hela人宫颈癌细胞的生长抑制作用显著增加,蓝莓花色苷组分由12种降为9种。对胃肠消化花色苷的降解过程推测发现,矢车菊素-3-葡萄糖苷降解形成槲皮素的过程与天竺葵素-3-葡萄糖苷降解成山柰酚的过程机制相同。

Changes in total polyphenols and anthocyanins contents,antioxidant capacity and anticancer effect,anthocyanins compositions of blueberry extracts before and after in vitro digestion were investigated.The degradation pathway of anthocyanins was speculated.The total polyphenols content and anthocyanins content were measured by Folin-Ciocalteu reagent method and pH differential method,respectively.The antioxidant capacity of the extracts before and after in vitro digestion was evaluated by the inhibition rate of liposomes,1-diphenyl-2-picrylhydrazyl(DPPH)and ABTS+free radicals scavenging abilities.Furthermore,the compositions of anthocyanins were analyzed using by high performance liquid chromatographyelectrospray ionization mass spectrometry(HPLC-EIS-MS).The results indicated that after in vitro digestion the content of total polyphenols increased by 47.21%,while that of anthocyanins decreased by71.82%,and the inhibition rate of liposomes,scavenging rates of DPPH and ABTS+free radicals increased by 38.45%,29.41%and 29.12%,respectively.Blueberry extracts could significantly inhibit the growth of HepG2 hepatoma cells,A549 lung cancer cells and Hela human cervical cancer cells.The varieties of anthocyanins decreased from twelve to nine.The degradation process of gastrointestinal anthocyanins revealed that the process of degradation of cyanidin 3-glucoside to form quercetin was similar to that of degradation of pelargonidin 3-glucoside into kaempferol.