碱性磷酸酶辣根过氧化物酶结合物在包虫病Elisa中对比应用

Application of Alkaline Phosphatase and Horseradish Peroxidase Conjugates on the Diagnosis of Hydatid Disease

在包虫病Elisa中对比应用了碱性磷酸酶及辣根过氧化物酶结合物。在40例中,0.1μg抗原/孔包板,阳性检出率分别为87.5％及85.0％;当抗原浓度降至0.03μg/孔时,阳性率分别为80％及75％。碱性磷酸酶底物无毒性,阳性率较辣根过氧化物酶法相对提高,具有一定的优点。

Both alkaline phosphatase (AKP)-joint anti Human IgG (AKP-AHG) and HRP-protein A applied to ELISA for the diagnosis of hydatid disease are described. In 40 cases, the positive rates were 87.5% and 85％ respectively with AKP-AHG and HRP-protein A conjugates (coated antigen 1.0μg/ml). Coated antigen as little as 0.3μg/ml decreased the positive rates to 80％ and 75％ respectively. The AKP method possesses the advantage of being nontoxic in regard to the enzyme substrate.