快速测定微量蛋白质的方法

The method of rapidly determing microgram protein

随着生化技术的日益发展,越来越需要对微量蛋白质进行快速定量。劳里(Lowry)蛋白质测定法是目前最经典的方法之一,但根据需要产生了一些改良法,而仍费时,操作也较繁琐,并有许多试剂干扰劳里法的测定结果。最重要的缺点是它不能用于测蛋白含量在10μg以下的样品,因此在实验工作中受到限制。

In this paper, a protein determination ethod which involves the binding of coomassie brillant blue G-250 to protein is described. It can be ust to determine micro-gram protein with microscanner and 96-well plates, The advantages of the method include: good reproduction (c.v=5％, n= 15), a little sample (20), simplicity and rapidity in processing (88 samples per hour). This method may increase the efficiency of work evidently, when a large amount of protein samples are determinated. This method can be used not only to determine the quantity of protein during the processes of laboratory separation and extraction, but also to determinate the proteins of almost all body fluids in the clinical diagnosis.