正常成人骨髓巨核细胞和其他有核细胞分类计数报告

Reports of megkaryocytes and other nucleated cells differential count in normal adult bone marrow aspiration

目的进一步探讨正常成人骨髓巨核细胞和其他有核细胞分类计数的参考值。方法骨髓液用EDTA-K2液抗凝,吸取5μl制成约2×3.5或2.5×3 cm(7 cm2)的厚薄均匀涂片,用光镜分类计数全片巨核细胞换算成涂片单位面积细胞数,其他有核细胞计数500只换算成百分比。结果巨核细胞在一个单位面积(1.5×3 cm,4.5 cm2)中为17~102(51.00±26.25)只,习惯涂片面积(7 cm2)为26~166(79.12±40.83)只,每1μl为5~33只。粒系细胞45.8~60.2(54.08±4.50)%,有核红细胞为17.0~33.2(25.46±4.64)%,粒红比值为1.4~3.4∶1(2.19±0.57∶1)。结论积累正常人骨髓巨核细胞定量计数和其他有核细胞参考值有助于提高细胞形态学的正确分析和诊断。

Objective To further explore the normal referential results of megkaryocytes and other nucleated cells differential count in normal adult bone marrow aspiration specimens. Methods Bone marrow aspiration were anticoagulated by EDTAK2,and 5 μl aspiration were drawn to make a about 2×3.5 or 2.5×3 cm （7 cm2） even thickness smear.megkaryocytes were counted in whole smear by microscope and calculated the quantities in per smear area,other nucleated cells were counted 500 and calculated the percent. Results The magkaryotytes in per smear area（1.5×3 cm,4.5 cm2） were 17～102（51.00±26.25）,in common use smear area（7 cm2） were 26～166（79.12±40.83）,in per μl were 5～33.The granulocytic cells number in total bone marrow nucleated cells were 45.8～60.2（54.08±4.50） %,the erythroblasts number were 17.0～33.2（25.46±4.64） %,G∶Eratio were 1.4～3.4∶1（2.19±0.57∶1）.Conclusion Accumulating normal referential results of megkaryotytes and other nucleated cells in normal adult bone marrow aspiration will help us to analyze and diagnose in morphology more correctly.