电泳法测定血清低密度脂蛋白胆固醇及临床应用

Quantitative determination of LDLC by agarose gel electrophoresis and its clinical application

目的对一种新型的琼脂糖凝胶电泳加胆固醇酶染色法定量分析低密度脂蛋白胆固醇[LDL-C]含量的方法进行评价,并探讨了与临床常用LDL-C均相测定法的相关性。方法采用Helena REP全自动快速电泳系统分离血清LDL-C,然后结合胆固醇酶染色法测定LDL-C的含量,分析了该法的精密度、准确度和干扰因素,以及正常参考范围,并将该法与临床常用的LDL-C均相测定法进行了比较。结果电泳法测定LDL-C批内CV为3.1%~4.5%、批间为3.7%~5.4%,检测线性范围为0.85~10.10 mmol/L,该法基本不受黄疸、溶血干扰,LDL-C的回收率93.7%~95.5%。电泳法(X)与LDL-C均相测定法(Y)测LDL-C的回归方程为Y=0.988X+0.206(r=0.989),参考范围为1.68~2.97 mmol/L。结论电泳法测定LDL-C的值与临床常用LDL-C均相测定法相关,电泳法能完全的分离出LDL-C带,克服均相测定时非低密度脂蛋白胆固醇反应引起的误差,并能同时分离测定VLDL-C、LDL-C、HDL-C和LP(a)-C,操作简便,给临床提供快速准确的实验数据。

Objective To evaluate a agarose gel electrophoresis for quantitative determination of Low Density Lipoprotein cholesterol and its clinical application. Methods Quantification of LDLC was performed by a new agarose gel electrophoresis method that allows the separation of LDLC by Hellen REP system and the determination of LDLC by enzymatic staining of cholesterol,The results of electrophoresis method were compared with the one of homogeneous method.The specimen of 135 health men was assayed by electrophoresis for the reference range. Results The inter and intra CV of electrophoresis method at low,middle,and high concentration specimens were 3.1%～4.5% and 3.7%～5.4%.The linearity was 0.85～10.10 mmol/L.Interference with bilirubin(<348 μmol/L),hemoglobin(<18 g/L) and triglyceride(<10mmol/L) was neglected.The recovery was 93.7%～95.5%.The values by this method(X) were closely correlated with those obtained by homogeneous method(Y) \.The reference range was 1.68～2.97 mmol/L. Conclusion The precision and accuracy of LDLC determination by the agarose gel electophoresis and enzymatic staining of cholesterol can meet the clinical demand.