类风湿关节炎患者淋巴细胞协同刺激分子和活化标志的表达及意义

Expression of costimulatory molecule and activation marker on lymphocytes with rheumatoid arthritis by flow cytometry

目的探讨淋巴细胞协同刺激分子、活化分子标志在类风湿关节炎(RA)致病机制中的作用。方法采用流式细胞仪检测RA患者外周血和关节滑膜液淋巴细胞协同刺激分子CD28/B7(CD80,CD86)、CD154(CD40L)/CD40和淋巴细胞活化标志 CD25、HLA-DR表达。结果:RA患者外周血B淋巴细胞CD86表达比正常对照组明显下降(P【0.01),B淋巴细胞CD40表达比正常对照组明显增多(P【0.05),而B淋巴细胞CD80表达,T淋巴细胞CD28、CD154表达及关节滑膜液T、B淋巴细胞协同刺激分子表达与正常对照组无显著性差异(P】0.05);同时RA患者外周血T、B淋巴细胞CD25表达比正常对照组明显增多 (P【0.01),关节滑膜液T、B淋巴细胞HLA-DR表达及B淋巴细胞CD25表达比正常对照组明显增多(P【0.01)。结论RA患者中存在淋巴细胞协同刺激分子和活化标志异常表达,这将为临床诊断治疗RA提供新的思路。

Objective To research the expression of costimulatory molecule and activation marker on lymphocytes in the patients with rheumatoid arthritis (RA) and to identify their role in the pathogenesis of RA.Methods Flow cytometry was used to detect the expression of costimulatory molecule CD28/B7(CD80,CD86) ,CD154(CD40L)/CD40 and activation marker CD25,HLA-DR on peripheral blood and synovial fluid lymphocytes in the patients with RA.Results Compared with control group,the expression of CD86 on peripheral blood B lymphocytes in the patients with RA was obviously decreased (P<0.01),the expression of CD40 was also significantly increased (P<0.05).No statistically significant differences in peripheral blood B lymphocytes expressing CD80,T lymphocytes expressing CD28,CD154 and in synovial fluid lymphocytes expressing costimulatory molecule between RA group and control group were observed,and there are no differences(P>0.05). Comparing with control group,the expression of CD25 on peripheral blood T and B lymphocytes in the patients with RA was obviously increased (P<0.01),the expression of CD25 on synovial fluid B lymphocytes and HLA-DR on synovial fluid T and B lymphocytes were also significantly increased(P<0.01).Conclusion The disorder expression of costimulatory molecule and activation marker on lymphocytes in the patients with RA may be play an important role in the pathogenesis of RA.These will give us a new idea on the diagnostic and therapeutic strategy of RA.