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微量离心柱法分离检测甲胎蛋白异质体对原发性肝癌诊断的意义

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摘要 目的探讨采用微量离心柱法检测甲胎蛋白异质体(AFP-L3)在原发性肝癌诊断中的意义。方法选择原发性肝癌(PHC)患者45例、肝硬化患者26例、慢性肝炎患者58例,采用预装有结合小扁豆凝集素(LCA)的琼脂糖微量离心柱分离血清AFP-L3,化学发光法检测AFP和AFP-L3,并计算AFP-L3在AFP中的含量比例,分析AFP-L3(%)升高者与良恶性肝病的关系。结果PHC患者血清中AFP-L3(22.9±9.3%)明显高于其它肝病患者(10.6±10.1%和4.8±3.5%),P值均【0.01,以AFP-L3(%)≥10%作为诊断标准,在已经确诊为肝癌的患者中灵敏度91.1%,与慢性肝病的鉴别诊断的特异性为92.8%;AFP-L3含量与血清AFP浓度及原发性肝癌癌灶大小无关。结论微量离心柱法检测甲胎蛋白异质体(AFP-L3)在PHC诊断,尤其是小肝癌的早期诊断和与低AFP含量肝病的鉴别诊断中具有重要的临床应用价值。
出处 《浙江检验医学》 2008年第4期32-33,共2页 Zhejiang Journal of Laboratory Medicine
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