IE抗原检测在HCMV感染中的诊断意义

Effectiveness of IE antigen assays for detecting HC MV infection

目的探索即刻早期抗原(immediate early antigen,IE抗原)检测在骨髓移植术后人巨细胞病毒感染中的诊断价值。方法回顾性分析骨髓移植术后160份外周血标本,根据外周血巨细胞病毒被膜抗原pp65的不同分为感染组和对照组,运用免疫组化法检测外周血人巨细胞病毒IE抗原和被膜抗原pp65。结果20名患者中,实验组16名(136份血标本),对照组4名(24份血标本)。160份外周血标本中,人巨细胞病毒pp65抗原阳性标本95份(59.38%),IE抗原阳性94份(56.75%)(P=0.91);IE抗原和pp65抗原术后检出时间分别为2.44±1.38周和3.17±1.16周(P=0.031)。结论IE抗原检测能发现早期特异性反应HCMV感染。

Objective The present research was performed to evaluate the IE antigen assays for detecting HCMV infection after transplantation of bone marrow.Methods 160 blood samples were collected from the recipients of transplantation of bone marrow.The patients were divided into an infection group and a control based on a peripheral blood pp65 antigen assay.CMV antigens were detected by immunohistochemical methods assay.Results 95 samples were positive by the pp65 antigen assay,94 samples were positive by the IE antigen assay.IE antigen appeared earlier(2.44±1.38 weeks post-transplantation)than pp65 antigen(3.17±1.16 weeks,P =0.031).Conclusion The IE antigen assay is a sensitive and specific diagnostic technique for rapidly and accurately detecting CMV infection.