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外排泵adeB基因表达与鲍曼不动杆菌泛耐药的关系 被引量：4

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摘要 目的探讨主动外排泵adeB基因在泛耐药鲍曼不动杆菌(Pandrug Resistance Acinetobacter Baumannii PRABA)耐药性中的作用。方法M-H琼脂稀释法检测临床分离PRABA对常用抗生素的最小抑菌浓度MIC,同时测定经外排泵抑制剂碳酰氰基-对-氯苯腙(CCCP)处理前后PRABA对常用抗生素的MIC值;PCR实时荧光RT-PCR检测外排泵adeB基因及其表达水平,PCR动力学经典算法:QR=(1+E)-△△CT,对基因表达进行相对定量分析。结果10株PRABA adeB基因阳性,2株全敏感鲍曼不动杆菌(PSABA)adeB基因阴性,PRABA菌株adeB基因mRNA的表达范围为2.5E+2~2.45E+3拷贝。结论PRABA菌株主动外排泵adeB基因高表达导致对常用抗生素泛耐药,甚至全部耐药。
作者 杨爱平 李向阳
机构地区 温州医学院附属第二医院
出处 《浙江检验医学》 2010年第1期7-10,共4页 Zhejiang Journal of Laboratory Medicine
关键词 鲍曼不动杆菌 adeB基因 外排泵 耐药
分类号 R446.5 [医药卫生—诊断学]
  • 相关文献

参考文献11

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共引文献16

同被引文献57

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引证文献4

二级引证文献12

浙江检验医学
2010年 第1期

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