慢性鼻-鼻窦炎患者细菌生物膜形成与细菌培养的相关性研究

Investigate on the relativity between the presence of bacterial biofilm and the results of bacterial culture in patients with chronic rhinosinusitis

目的探讨细菌生物膜在慢性鼻-鼻窦炎患者鼻黏膜中的表达与细菌培养的相关性。方法实验组获取90例慢性鼻-鼻窦炎患者手术黏膜样本,对照组获取5例鼻中隔偏曲患者及10例健康志愿者钩突黏膜样本,所有样本均行细菌培养及扫描电子显微镜检查。分析细菌生物膜表达与细菌培养的相关性。结果 90例实验组患者中,64例观察到细菌生物膜的存在(阳性率71.1%)并且其中有60例细菌培养阳性,检出率最高的细菌是表皮葡萄球菌占41.7%(25/60)。另26例实验组患者及15例对照组患者均未观察到细菌生物膜,也未培养出需氧或厌氧菌。细菌培养与细菌生物膜间的Pearson相关系数为0.901(P=0.000)。结论慢性鼻-鼻窦炎患者鼻黏膜中细菌生物膜表达与细菌培养阳性结果呈高度相关。

Objective To investigate the relativity between the presence of bacterial biofilm and the results of bacterial culture in patients with CRS.Methods Ninety patients with chronic sinusitis were enrolled in the study goup.The control group was 5 patients with deviation of nasal septum and 10 healthy volunteers.Mucosa of uncinate process or near the ostium of the maxillary sinus was obtained during endoscopic sinus surgery.The specimens were divided into 2 fragments:the first was processed for bacterial cultures,and the second was subjected to scanning electron microscopy.Statistical analysis was performed.Results The scanning electron microscopy analysis showed bacterial biofilms in 64 of the 90 patients with chronic sinusitis.A marked destruction of the epithelium and cilia was observed in samples positive for biofilms.The positive rate of bacterial culture in the study group was 66.7%(60/90).No bacterial biofilm and bacterium was detected in the control group.Pearson relation analysis showed a statistically association between bacterial biofilm and bacterial culture in CRS.Conclusion Bacterial biofilms were demonstrated to be present in CRS.Positive result of bacterial culture was highly correlated with the presence of bacterial biofilm in CRS.