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田基黄对防治内毒素血症性肝损伤的实验研究~

The protective effect of hypericum japonicum thunb against LPS-induced acute liver injury in mice
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摘要 目的探讨田基黄对内毒素血症引起的肝损伤的防治作用。方法对Wister大鼠用不同剂量的田基黄水煎剂或联苯双酯进行灌胃,1次/d,连续7 d。于最后一次给药1小时后,空白组尾静脉注射生理盐水,其余各组尾静脉注射LPS 5 mg/kg。在用药24小时后下腔静脉采血做实验室指标检测,取肝组织做病理学检查。结果通过肝组织切片,LPS模型组可见脂肪变性,肝细胞灶性及带状坏死,肝窭结构破坏,汇管区炎细胞浸润。而田基黄组脂肪变性少见,偶有点状坏死,无明显炎细胞浸润。表明田基黄能减轻LPS导致肝组织病理损伤,同时抑制LPS引起的小鼠血清ALT活性的比高,抑制NO,TNF-α和IL-6的过量分泌。结论田基黄时内毒素引起的肝损伤有一定的防治作用。 Objective To study the protective effect of Hypericum japonicum Thunb against LPS-induced acute liver injury in mice and its mechanism.Methods Mice were feeded with Hypericum japonicum Thunb and bifendate respectively,one time per day,continuing with one week.Then all mice were injected with LPS.After 24 hours,ALT,NO,TNF-aand IL-6 were determined and pathological change of liver was observed.Results Hypericum japonicum Thunb could significantly decrease the elevation of ALT,NO,TNF-aand IL-6 induced by LPS,and ameliorate the pathological injury of liver.Conclusion Hypericum japonicum Thunb shows protective effect against experimental liver injury in mice.Restraining produce of the serum NO,TNF-aand IL-6 may be one of its mechanisms.
出处 《浙江检验医学》 2013年第1期24-26,共1页 Zhejiang Journal of Laboratory Medicine
基金 浙江省中医药科技计划项目资助(2010ZB084)
关键词 田基黄 内毒素 肝损伤 Hypericum japonicum thunb Endotoxin Liver injury
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