多波长系数法测定复方氯霉素醇溶液中3组分的含量

Determination of the Contents of Three Ingredients in Compound Chloramphenicol Tincture by Multiˉwavelength Coefficient Spectrophotometry

目的 :建立不经分离同时测定复方氯霉素醇溶液中氯霉素、水杨酸、甲硝唑含量的方法。方法 :采用多波长系数法测定3主药的含量 ,测定波长分别为277 5、297 0、317 0nm。结果 :平均回收率氯霉素、水杨酸、甲硝唑分别为 (100 22±0 74) %、(99 25±1 54) %、(99 79±1 92) %。结论 :该方法简便、快速、准确 ,适用于医院中多组分制剂的常规分析。

OBJECTIVE:To establish a method for simultaneously determining chloramphenicol,salicylic acid and metroni_ dazole in compound chloramphenicol tincture with no need for separation.METHODS:The concentrations of three main inˉgredients were determined by multiwavelength coefficient spectrophotometry,the detecting wavelengths being277.5,297.0and317.0nm respectively.RESULTS:The average recoveries of chloramphenicol,salicylic acid and metronidazole were(100.22±0.74)%,(99.25±1.54)%and(99.79±1.92)%respectively.CONCLUSION:This method is simple,rapid and accurate.It is suitable for the determination of multicomonent preparation in hospital routine analysis.