妊娠滋养细胞肿瘤中VEGF CyclinD1的表达及意义

The expression and significance of VEGF, Cyclin D1 in gestational trophoblastic tumor

目的 探讨血管内皮生长因子 (VEGF)、细胞周期素D1(CyclinD1)在妊娠滋养细胞肿瘤发生、发展及估计预后中的作用。方法 采用免疫组化S -P法检测VEGF、CyclinD1在正常早孕绒毛及妊娠滋养细胞疾病中的表达。结果 从正常绒毛→葡萄胎→侵蚀性葡萄胎→绒毛膜癌 ,VEGF、CyclinD1表达率逐渐升高。妊娠滋养细胞肿瘤组与正常绒毛、葡萄胎组比较 ,VEGF、CyclinD1的表达率有显著性差异 (P <0 0 5 ,P <0 0 0 1) ;随肿瘤临床期别的增加 ,VEGF、CyclinD1表达率逐渐增高 ,Ⅲ～Ⅳ期与Ⅰ～Ⅱ期比较 ,有非常显著性差异 (P <0 0 0 5 ) ;VEGF与CyclinD1的表达呈正相关 (r =0 483,P <0 0 0 5 )。 结论 VEGF、CyclinD1对预测滋养细胞的恶变 ,判断预后有一定的参考价值。

ObjectiveTo explore the role of vascular endothelial growth factor (VEGF) , Cyclin D1 in oncogenesis, development and prognosis of gestational trophoblastic tumor . MethodsThe expression of VEGF, Cyclin D1 in normal chorion of early gestation, hydatidiform mole and invasive mole, choriocarcinoma was detected by immunohistochemistry test ( SP method). ResultsFrom normal chorion of early gestation to hydatidiform mole,invasive mole and choriocarcinoma, VEGF, Cyclin D1 showed an ascending tendency. The positive rate of VEGF and Cyclin D1 in gestational trophoblastic tumor group was higher than that of the normal chorion of early gestation group and hydatidiform mole group (P<0.05 P<0.001). Follow the raise of clinical phase in gestational trophoblastic tumor, the expression of VEGF and Cyclin D1 were gradually increased. The positive rate of VEGF and Cyclin D1 was significantly higher in stage Ⅲ and stage Ⅳ than those in stage Ⅰand stage Ⅱ(P<0.005). There was positive correlation between VEGF and Cyclin D1(r0.483 P<0.005).ConclusionVEGF, Cyclin D1 are of value in predicting the malignant change and prognosis of the trophoblastic cells. re higher than that of it in local matrimonial physical examination.Key words:sexually transmitted diseases;premariral examination;foreign nationalsStudy on Holmium-chitosancomplex in normal brain of the rats$$$$SUN Jing-he1, JOH Chul-wo2, AHN Young-hwan2, SHIM Chul2, PARK Kyung-bae2, CHO Kyung-gi3(1.Department of Neurosurgery, Affiliated Hospital of Yanbian University College of Medicine, Yanji 133000, Jilin, China;2.School of Medicine, Ajou University, Korea;3.Korea Atomic Energy Research Institute, Korea)ABSTRACT:OBJECTIVETo study the effects of 166 Holmium and 166 Holmium-chitosan complex(166Ho-CHICO) for the normal rat brain and to determine its sublethal optimal dose.METHODSConsecutive 117 rats were divided into 4 groups of 166 Ho treated(n=50), 166 Ho-CHICO treated(n=57), saline treated(n=5) and chitosan treated(n=5) group. 166 Ho and 166 Ho-CHICO were injected stereotactically with 3.7, 7.4, 11.1, and 14.8MBq/20μL using a automated micro injector. Nuclear, histopathological and hematological studies were performed in 10 animals per group at 1 day, 3 days, 7 days, 1 month and 3 months after injection.RESULTSAn infiltration of inflammatory cells and necrotic changes were noted in radioisotope treated group at 1 week after injection. A wedge-tissue defect due to necrotic in 166 Ho treated group and a cystic defect lined with reactive astroglial cell in 166 Ho-CHICO treated group at 3 months after injection. 166 Ho was leaked out and caused necrotic lesion on the superficial cortex; but 166 Ho-CHICO treated group did not show this feature. As the dose of 166 Ho increased, mortality rate was increased significantly. Difference of mortality rate of the 166 Ho-CHICO group was signifiacantly higher than that of 166 Ho treated group in a dose of 14.8MBq/20μl/300g. There were no detectable radioactivities by the leakage and extravasation from the injected site of brain on Gamma camera at 1 hour, 24 hours and 48 hours after injection. There were no detectable abnomal appearance of other organ including spleen, liver and kidney.CONCLUSION166 Ho-CHICO is not leaked out from the injection site but 16