神经元特异性稀醇化酶和S-100蛋白免疫组化染色在先天性巨结肠诊断中的应用

Application of Neuron-Specific Enolase and S-100 Protein in Diagnosis of Hirschsprung's Disease

目的 :探讨神经元特异性稀醇化酶(NSE)和S -100蛋白免疫组化染色技术诊断先天性巨结肠(HD)的价值。方法 :对41例临床疑似HD病人行肠黏膜活检 ,采用NSE和S -100蛋白免疫组化链酶卵白素(SP)法染色。结果 :神经节细胞对NSE呈强阳性反应 ,阳性细胞呈深棕色 ,而雪旺细胞和周围胶质细胞对NSE呈弱阳性反应。神经节细胞对S -100蛋白呈阴性反应 ,雪旺细胞和胶质细胞对S -100蛋白呈强阳性反应。这两种抗体染色结果相互对照 ,使神经节细胞极易辨认。41例病人中35例确诊为先天性巨结肠 ,全部经手术后全层病理检查证实 ,诊断符合率100 %。其余6例诊断为其他肠道疾病 ,后经临床观察和药物治疗证实不是先天性巨结肠。结论 :NSE和S

Objective:To investigate the applied value of neuron_specific enolase(NSE)and S_100protein in diagnosis of Hirschsprung's disease(HD).Methods:The intestinal mucomembranous biopsy of41cases of dubiˉous HD was made with SP immunohistochemical staining method.Results:Neurons were strongly positive to NSE and their positive cells were dark brown;while Schwann cell and gliocyte were weakly positive.Neurons were negative to S-100protein,while Schwann cell and gliocyte were strongly positive.When the two staining results were compared each other,neurons were clear.Among the41cases,35cases were diagnosed as HD,and then the diagnosis was confirmed by postoperatively pathological examination.The other6cases were diagnosed as other intestinal diseases through clinical observation and medication.Conclusion:The expression of NSE and S_100protein immuno_staining are of an important significance to the confirmed diagnosis of HD.