高效液相色谱测定大鼠血浆儿茶酚胺的含量

To detect the contents of mice plasma catecholamine by the high efficient liquid chromatography

目的 :改进高效液相色谱配电化学检测器检测血浆儿茶酚胺 (CA)类物质含量的技术。方法 :用不同的流动相条件 ,在体外用外标法对大鼠血浆 CA含量进行检测。结果 :色谱条件以流动相用双蒸水配制 ,脱气 3 0 min,p H 2 .8～ 3 .0 ,检测电压 0 .6V,流速 0 .6m l/ min外标法为理想检测条件。去甲肾上腺素 (NE)最小检测极限 1.0 0 3 ng/ ml,肾上腺素 (E)最小检测极限 1.2 1ng/ ml。结论 :本文方法简便、快速、可靠 ,可用于科研测定血浆 NE和

AIM:To improve the technique of the high efficient liquid chromatography of mice plasma catecholamine to study the changes of plasma catecholammine (CA) content. METHODS:To measure the plasma CA content of bound stress mice labeled in vitro by the method of high efficient liquid chromatography electrochemical detector. RESULTS:Chromatography conditions were made with the flow phas double steam water and degassed completely,pH 2.8~3.0,detecting voltage 0.6 V,flow speed 0.6 ml/min. The detected smallest limit of norepinephrine(NE) is 1.003 ng/ml,the detected smallest limit of epinephrine(E) is 1.21 ng/ml. This way is very idea.CONCLUSION:This measure method is simple, swift and confident;it can be used to detect the plasmas NE and E in clinic and basic research.