白术对胃粘膜上皮细胞功能作用的实验研究

THE EXPERIMENT STUDY TO EFFECT OF LARGE BIGHEAD ATRACTYLODES ON HUMAN GASTRIC MUCOSA CELLS FUNCTION

目的 :研究白术治疗慢性胃病的机理。方法 :用白术提取液与胃粘膜细胞共同培养 ;3H -胸腺嘧啶核苷掺入法观察胃粘膜细胞增殖状况 ;改良李氏法测定胃蛋白酶含量 ,pH仪测定胃酸分泌量。结果 :0 .5 %和 1 %白术浓度组cpm值显著高于对照组和 0 .2 5 %浓度组 (P <0 .0 5～ 0 .0 1 ) ,但 0 .5和 1 %浓度组之间无显著性差异 (P >0 .0 5)。 0 .5 %和 1 %白术浓度组胃蛋白酶分泌量与对照组和 0 .2 5 %浓度组比较差异显著 (P <0 .0 1 )。实验组中各组 pH值低于对照组 ,但无统计学意义 (P >0 .0 5)。结论 :白术能促进胃粘膜细胞的增殖 。

Objective:To study the mechanism of the effects of large Bighead Atractylodes on treating gastrc disease. Methods:Human gastric mucosa epithelium were cultured in vitro, and the effect of large Bighead Atractylodes were observed on human gastric mucosa cells growth, DNA synthesis (detected by 3?H-TdR method), pepsin secretion detected by improved Li's method) and acid secretion(detected by pH meter).Results:The cpm in groups of 0.5% and 1% the drug was significantly higher than control group and 0.25% group ( P <0.05～0.01). But, there are no significantly higher between groups of 0.5% the drug and 1% one ( P >0.05%). The amounts of pepsin in groups of 0.5%,1% of the drug were significantly higher than control group and group of 0.25% ( P <0.05).The PH value in groups 0.5%, 1% of the drug were lower than control group and 0.25% group, but there are no statistical significance.Conclusion: These results showed that large Bighead Atractylodes could stimulate the growth of gastric mucosal cells and pepsin secretion.