种源对单级自养脱氮系统同步构建影响研究

Effects of seed sludge on the simultaneous construction of single-stage autotrophic nitrogen removal system

针对单级自养脱氮微生物系统异步分期构建周期长、过程复杂、接种污泥难于获取等问题,开展单级自养脱氮系统同步构建研究。以SBBR反应器为对象,分别以好氧活性污泥、下水道底泥、脱水污泥作为接种污泥,系统研究种源对单级自养脱氮系统同步构建的影响,并采用PCRDGGE技术探讨了系统微生物种群结构的变化。结果表明:反应器在温度为(30±1)℃、初始氮负荷为0.025kgN/(m^3·d)的条件下,进水NH3-N浓度由最初的100mg/L逐渐提升至2 150mg/L,连续运行153~190d,各反应器的去除负荷均达到0.50kgN/(m^3·d)以上,NH_3-N和TN去除率分别为99.4%和91.8%以上,接种污泥种类对单级自养脱氮系统构建有显著影响。采用脱水污泥作为接种污泥,单级自养脱氮系统构建速度最快为153d,其来源广泛可作为工程化应用的接种污泥。PCR-DGGE研究表明:在系统构建过程中微生物群落结构及优势种群发生了变化,与相对应的接种污泥样品相比,成功构建稳定运行后系统内微生物种群分属于另一不同集群。

Aiming at the problem of single-stage autotrophic nitrogen removal microbial system that the long asynchronous construction period,complex process,anddifficulty to obtain inoculation sludge,the simultaneous construction of single-stage autotrophic nitrogen removal system was investigated using SBBR reactor.The effects of seed sludgeon the simultaneous construction of single-stage autotrophic nitrogen removal system were studied by using aerobic activated sludge,sewer sediment and dewatered sludge as inoculated sludge.PCR-DGGE was used to study the changes in microbial community structure.The results showed that when the concentration of NH3-N in the reactor was gradually increased from 100 mg/L to 2 150 mg/L after 153~190 d,the removal load of each reactor reached 0.5 kgN/(m3·d),and TN removal rate were 99.4% and 91.8%,respectively,under the conditions of(30±1)℃,0.5 kgN/(m3·d)of initial nitrogen loading.Inoculation ofsludge species has a significant effect on the construction of single-stage autotrophic nitrogen removal system.Single-stage autotrophic nitrogen removal systemwas constructed at the fastest speed of 153 d,using dehydrated sludge as inoculation of sludge,which can be widely used as engineering applications for inoculating sludge.The results of PCR-DGGE showed that the microbial community structure and dominant population changed during the system construction.Compared with the corresponding inoculated sludge samples,the microbial population after successful operation was distributedin another cluster.