线叶金雀花与藏红花水提取物抗疲劳的作用研究

Anti-fatigue effects of aqueous extracts from Xianye Jinquehua and Zanghonghua

目的研究线叶金雀花与藏红花水提物的抗疲劳作用。方法先将32只小鼠随机分为空白对照组、线叶金雀花组、藏红花组,灌胃给药,1次/d,14 d后进行负重游泳实验。然后,将46只小鼠随机分为空白对照组、线叶金雀花+藏红花低剂量组,灌胃给药,1次/d,14 d后进行负重游泳实验并检测血清乳酸含量。根据上述实验结果,将100只小鼠随机分为空白对照组、线叶金雀花+藏红花低剂量组、线叶金雀花+藏红花中剂量组、线叶金雀花+藏红花高剂量组,灌胃给药14 d后,检测肝糖原、肌糖原的含量。结果单独灌胃线叶金雀花、藏红花水提物均不能增加小鼠负重游泳时间,低剂量线叶金雀花与藏红花合用能够延长小鼠负重游泳时间(P<0.05),减少血清乳酸含量(P<0.05)。与空白对照组相比,线叶金雀花+藏红花低、中、高剂量组均能增加小鼠肝脏糖原和肌肉糖原含量(P<0.05或P<0.01)。结论线叶金雀花、藏红花水提物单独给予不具有抗疲劳作用,而二者联合则具有抗疲劳作用。

Objective To study the anti-fatigue effects of aqueous extracts from Xianye Jinquehua(Rooibos,Aspalathus Linears)and Zanghonghua(Saffron,Crocus sativus L.)through classic behavior test(loaded swimming)and detections of blood lactic acid,liver glycogen and muscle glycogen.Methods At first 32 mice were randomly divided into blank control group,Xianye Jinquehua group and Zanghonghua group,and were orally given corresponding medicinals once a day.The test of loaded swimming was conducted in all groups after 14 d.Then 46 mice were randomly divided into blank control group and low-dose XianyeJinquehua+Zanghonghua group,and were orally given corresponding medicinals once a day.The test of loaded swimming and detection of blood lactic acid were conducted in all groups after 14 d.According to above test results,100 mice were randomly divided into blank control group,low-dose Xianye Jinquehua+Zanghonghua group,middose Xianye Jinquehua +Zanghonghua group and high-dose Xianye Jinquehua +Zanghonghua group.After oral medication for 14 d,the content of liver glycogen and muscle glycogen was detected.Results The time of loaded swimming was not improved in Xianye Jinquehuagroup and Zanghonghua group,and it was prolonged(P<0.05)and content of blood lactic acid was decreased(P<0.05)in low-dose Xianye Jinquehua+Zanghonghua group.The content of liver glycogen and muscle glycogen was increased in low-dose Xianye Jinquehua+Zanghonghua group,mid-dose Xianye Jinquehua+Zanghonghua group and high-dose Xianye Jinquehua+Zanghonghua group compared with blank control group(P<0.05 or P<0.01).Conclusion The single administration of single Xianye Jinquehua or single Zanghonghua has no anti-fatigue effect,while combination of them has this effect.