摘要
快速发展的RNAi(RNA interference)技术为选择性抑制特异性基因的表达提供了有利的工具。RNAi是外源的dsRNA(double strand RNA,>19bp)进入细胞后,激活细胞内自然存在的加工活性,引起对侵入的外源dsRNA及具有同源序列的单链RNA降解(包括内源性的mRNA)的现象。RNAi技术目前多作为基因功能研究的有利工具,随着RNAi分子机制研究的不断深入,相信它将成为最有潜力的基因干预治疗方法。
The rapidly developing RNAi interference (RNAi)methodologies hold the promise to selectively inhibit gene expression. RNAi is a cellular phenomenon that when exogenous double strand RNA molecule of greater than 19 duplex nucleotide, the innate cellular process is activated, causing the degradation of not only the invading dsRNA molecule, but also single-stranded RNAs of indentical sequences, RNAi is now an powerful instrument for functional genomic analyses. Along with the deepening investigation of molecular mechanism of RNAi, it will be a potentially useful method in highly specific dsRNA based gene-silencing therapeutics.
出处
《生命科学》
CSCD
2003年第1期7-11,共5页
Chinese Bulletin of Life Sciences
基金
国家自然料学基金(39825113,30000067)
军队(01Z080)资助
关键词
RNAI
基因沉默
干预治疗
RNAi
gene silence
therapeutic intervention
