生长素与乙烯在沙田柚上胚轴不定芽再生中的作用

Roles of auxin and ethylene in direct bud regeneration from epicotyl of Citrus grandis(L.)Osbeck cv. Shatian Yu

结果表明,6 BA只能诱导较低频率的不定芽再生,IBA的加入可以促进不定芽的再生。从再生率与单位外植体再生芽数综合考虑,以高浓度IBA(1.5mg·L 1)与低浓度6 BA(0.5mg·L 1)配合的效果最佳,加入生长素极性运输的调节剂TIBA与Flavone可进一步提高不定芽的再生频率。乙烯抑制上胚轴不定芽的再生,而乙烯生理作用的拮抗剂Ag+与生物合成抑制剂AOA、Co+可以显著提高不定芽再生频率。水杨酸(SA)也抑制不定芽的再生。

The roles of auxin and ethylene in direct bud regeneration from epicotyl of Citrus grandis(L.)Osbeck cv. Shatian Yu were investigated. The results showed that 6BA could not induce bud regeneration efficiently. The addition of IBA could promote it synergistically. The best results was obtained with the combination of 1.5 mg·L1 IBA and 0.5 mg·L1 6BA in terms of regeneration frequency and regenerated shoot number per explant. The auxin polar transport regulators,TIBA and Flavone,could further improve regeneration frequency. Ethylene inhibited shoot regeneration,while its antagonist,Ag+,and synthetic inhibitors,AOA and Co+,could significantly improve regeneration frequency. SA also inhibited shoot regeneration.