中华水韭(Isoetes sinensis)等位酶分析的初步研究

Preliminary Study on Allozyme Analysis for Isoetes sinensis Palmer

采用超薄平板聚丙烯酰胺等电聚焦技术对珍稀濒危植物中华水韭 (Isoetes sinensis Palmer) 72个样品的等位酶遗传变异进行了初步研究。从 2 4种酶系统中筛选 ,得到了 13种酶系统可用于该植物的遗传变异分析 ,其中 7种酶系统在中华水韭的迁地保护居群中检测出 16个位点 ,共 2 7个等位基因 ,12个位点表现出多态性。遗传多样性初步分析表明 :中华水韭具较低的等位酶遗传多样性。研究结果为中华水韭的综合保育及居群恢复、重建提供了初步的依据。中华水韭遗传变异的全面研究将在进一步改善等位酶方法的基础上 ,采用其它更为灵敏的分子标记方法做深入检测。

The allozymic diversity was preliminarily investigated for 72 samples of rare and endangered species Isoetes sinensis Palmer, using isoelectric focusing in thin-layer polyacrylamide slab gels. 24 enzyme systems were initially screened, resulting 13 workable enzyme systems. A total of 27 alleles of 16 loci were detected from 7 well-resolved enzymes. Although 12 loci were observed to be polymorphic in ex situ conserved populations at Wuhan Botanical Garden, the overall genetic diversity is low in I. sinensis. The study provided preliminary evidence that a comprehensive conservation and restoration program is needed for sustainable conserving and recovery of natural I. sinensis populations. A further study of I. sinensis population and conservation genetics is urgently needed by employing more sensitive molecular methods.