假单胞菌XN-1硝基苯降解性质粒的提取及研究

THE ISOLATION AND RESEARCH OF NITROBENZENE DEGRADATIVEPLASMID FROM PSEUDOMONAS XN-1

假单胞菌XN 1对有机污染物硝基苯具有降解性 ,并且对氨苄青霉素具有抗性。检测和提取了假单胞菌XN 1细胞内的质粒 ,得到了一个约 2 2kb大小的质粒pXN 1。质粒消除实验证实这个质粒与硝基苯降解性有关 ,而与抗生素抗性无关。XN 1和其自发突变株XN 1 2和XN 1 3特性的差异和质粒检测的差异之间存在对应关系 ,并且得到了一个比 pXN 1小几个kb的衍生质粒 pXN 1 3。

Pseudomonas XN 1 has the ability of degradation for organic pollutant nitrobenzene, and the resistance to ampicillin In this paper, the plasmid was detected and isolated from the cell of Pseudomonas XN 1, and plasmid pXN 1 whose size is about 22 kb was gained The experiment of plasmid elimination confirmed that the plasmid has some relation to the ability of nitrobenzene degradation, while it is irrelevant to the resistance to ampicillin The feature difference between XN 1and its spontaneous mutants was corresponding to the difference of the plasmids detection, and a derivative plasmid pXN 13 was obtained from mutant XN 13 which is a few kb smaller than pXN 1