曲霉液体发酵产原果胶酶的条件优化研究

STUDY ON THE OPTIMUM FERMENTATION CONDITION FOR PRODUCTION OF PROTOPECTINASE BY ASPERGILLUS SP.

研究了碳源、氮源、金属离子及表面活性剂等对菌株 (Aspergillussp )XZ 1 31产原果胶酶的影响。果胶类物质是该菌株产原果胶酶所必需的诱导物。以 (NH4 ) 2 SO4 和(NH4 ) 2 HPO4 作为氮源时 ,产酶较高 ,达到 30 0U/mL。钙离子及Tween 2 0均能促进该酶的产生。通过正交试验优化得出该菌株产酶的最佳培养基配方为 :桔皮粉 1 g ,(NH4 ) 2 SO4 2 g ,CaCl2 0 0 1 5 g ,Tween 2 0 0 2mL ,KH2 PO4 3 8g ,K2 HPO4 ·3H2 O 0 2 g ,水 1 0 0mL ,pH 6 5。

Effects of carbon resource, nitrogen resource, metal irons and surface detergents on the production of pro topectinase by strain Aspergillus sp XZ 131 were studied The results showed that pectin substances were essential for the strain to produce protopectinase The enzyme activity reached to 300 U/mL, when(NH 4) 2SO 4 and(NH 4) 2HPO 4 were used as nitrogen resource Ca 2+ and Tween 20 were able to enhance the production of the enzyme The optimum composition of the medium was citrus peel powder 1g,(NH 4) 2SO 4 2g,CaCl 2 0 015g,Tween 20 0 2mL,KH 2PO 4 3 8g,K 2HPO 4·3H 2O 0 2g,H 2O 100mL,pH 6 5。