HFRS病毒特异性抗体与淋巴细胞亚群检测的意义

Significance of Detecting Specific Andtibody and Lymphocyte Subsets of Hemoffhagic Fever with Renal Syndrome Virus

研究了肾综合征出血热病毒的特异抗体与淋巴细胞亚群检测对临床诊断的意义。用ELISA法对 30例临床诊断肾综合征出血热患者进行特异性IgM抗体检测 ,进而用流式细胞仪对IgM抗体阳性患者进行淋巴细胞亚群测定。正常对照 30例 ,均为健康人。肾综合征出血热患者特异性IgM抗体阳性 2 2例 ,检出率为 73 .33 % ,正常对照标本均未检出病毒特异性IgM抗体。肾综合征出血热患者CD4 +细胞百分比较正常对照组降低 ,CD8+细胞百分比较正常对照组增高 ,CD4 +/CD8+比值下降或倒置。肾综合征出血热病毒的特异性IgM抗体特异性强 ,可作为该病早期临床诊断的重要依据。肾综合征出血热患者的免疫功能明显下降 ,对于判定疗效和疾病预后等具有参考价值。

To study the significance of clinical diagnosis by detectining specific antibody and lymphocyte subsets of hemorrhagic fever with renal syndrome virus.To detect specific IgM antibody and calculate the detecting rate from 30 cases of HFRS patients by ELISA(enzyme linkes immunoadsorbent assay).The lymphocyte subsets of positive patients were detected by FCM(flowcytometer).30 cases of healthey control were detected in this experiment.The detecting rate of special IgM antibody in HFRS' group was {73.33}%(22/30).Control group was negative.Compared with control group,the patients' percentage of lymphocyte CD-4 was lower,and on the contrary lymphocyte CD-8 was higher. Special antibody of HFRS has great significance on early diagnosis,because the IgM antibody has great specialty.The descending of inversion of the ratio of CD-4 and CD-8 suggests that the patients' immune function lowered,and has referent value for clinical diagnosis.;