利用定量杂交方法对几个人工和环境微生物样品的分析研究

Characterization of several synthetic and environmental microbial samples by quantitative hybridization.

利用真细菌和真核生物两个域特异性探针 ,初步建立起 16SrRNA定量杂交的方法 ,并对几个人工RNA样品 (由提取自S .cerevisiae和P .fluorescens纯培养细胞的RNA按一定比例混和而成 )和一个提取自垃圾填埋场渗滤液的RNA样品进行了初步的定量分析 .结果表明该方法能从域的水平上对这些样品的组成作较精确的分析 。

Limitations of traditional techniques based on selective enrichment and pure culture isolation make it difficult to precisely characterize the natural microbial ecosystems. Molecular techniques are now being developed and used to address these limitations. Group specific 16S rRNAs targeted oligonucleotide probes of different phylogenetic levels are increasingly used to identify and quantify the microbial members in complex environmental samples. Two domain specific probes were used in this study to elementarily characterize the defined mixtures of RNAs extracted from pure culture (synthetic samples) and a RNA sample obtained from landfill leachate (environmental sample). The results demonstrated that 16S rRNA quantitative hybridization provided an excellent estimation of domain level community composition of these samples, and thus, had a huge potential of usefulness in microbial ecology studies.