离子色谱法同时测定果汁中低级脂肪酸

以邻苯二甲酸氢钾和邻苯二甲酸混合水溶液为淋洗液,用阴离子交换柱直接电导检测,建立了果汁中四种低级脂肪酸同时分析的方法,考察了影响分离的因素。当邻苯二甲酸氢钾和邻苯二甲酸的浓度分别为0.75mmol/L和0.5mmol/L(pH=3.97)时,分离效果良好,各组分的相对标准偏差均在1.4%～2.8%之间。

An ion chromatog raphic method for the simultaneous analysis of low -g rade fatty acids including formic acid,acetic acid,propionic acid and butyric acid in fruit juice was developed.An anion exchang e column Shim -pack IC -A1(4.6mm i.d.×10cm)and a conductitivity detector were used.A mixed eluent of 0.75mmol /L potassium hydrog en phthalate and0.5mmol /L phthalic acid(pH=3.97)was used in this work.The4fatty acid in fruit juice were separated effectively.The precision of the method was investig ated and the relative standard deviations were from1.4%to2.8%(n=6).