重型乙型肝炎与无症状HBsAg携带者免疫相关基因表达差异的研究

The difference of immune-related gene expression between patients with fulminant hepatitis B and asymtomatic surface antigen carriers by cDNA microarray

目的 :应用基因芯片研究重型乙型肝炎 (FHB)与无症状HBsAg携带者 (ASC)外周血单个核细胞 (PBMC)免疫相关基因表达差异。方法 :应用含 8192条人体cDNA的微阵列芯片和来自外周血单个核细胞标记的cDNA ,分析了 10例重型乙肝和 10例无症状HBsAg携带者基因表达谱。通过应用GenePix 4 0 0 0B扫描仪和ImaGene3 0分析软件比较Cy5标记的FHB来源cDNA与Cy3标记的ASC来源cDNA的杂交结果 ,获得个体基因的相对表达比值。结果 :在 8192个基因中 ,初筛出 2 1个(0 2 5 % )表达差异达 2倍以上的免疫相关基因。结论 :在乙型肝炎病毒 (HBV)感染机体致慢性重型肝炎过程中 ,全面改变了宿主细胞内免疫相关基因表达 ,这些差异表达基因可能与慢性重型肝炎的发生有关。

Objective:To study (peripheral blood mononuclear cells)immune related gene expression difference in PBMC between patients with FHB(fulminant hepatitis B) and ASC (Asymtomatic surface antigen carriers) by cDNA microarray Methods:Microarrays consisting of 8 192 human cDNAs were applied to analyze gene expression using labeled PBMC cDNAs prepared from 10 FHB and 10 ASC Relative expression ratios of individual genes were obtained by comparing hybridization of Cy5 labeled cDNA from FHB and Cy3 labeled cDNA from ASC by scanning on a GenePix 4000B followed by image analysis using ImaGene3 0 software Results:Out of 8 192 genes,21 immune related genes were identified as differentially expression Conclusion:These results suggest that Hepatitis B virus infection alters expression of a broad array of immune related genes during developing into FHB ,which might play a role in pathogenesis of FHB