DNAM-1在系统性红斑狼疮患者T淋巴细胞的表达研究

Expression of DNAM-1 on T cells subsets from patients with systemic lupus erythematosus

目的 :研究DNAM 1在SLE患者外周血T淋巴细胞亚群上的表达 ,以阐明DNAM 1抗原在SLE患者体内活化作用以及与SLE发病的关系。方法 :31例SLE患者和 30例健康志愿者外周血单个核细胞 ,在PHA刺激培养 72小时后 ,三色荧光标记的单克隆抗体染色 ,利用流式细胞仪测定T淋巴细胞亚群膜表面DNAM 1抗原的表达。同时检测SLE患者抗dsDNA抗体、C3和C4 补体 ,疾病活动性用SLEDAI记分。结果 :SLE患者CD+4、CD+8淋巴细胞上DNAM 1表达率均高于正常对照组 (P<0 0 1) ;活动期SLE组CD+ 4、CD+ 8细胞上DNAM 1表达均高于正常对照组和静止期SLE组 (P <0 0 1) ,而静止期SLE组与正常对照组无显著性差异 (P >0 0 5 ) ;SLE患者CD+8细胞DNAM 1表达与SLEDAI、抗dsDNA抗体之间呈显著正相关 (P <0 0 1) ,与C3和C4 补体水平呈明显负相关 (P <0 0 5 ) ,CD+4细胞DNAM 1表达与SLEDAI、抗dsDNA抗体、C3和C4 补体之间无明显相关性 (P >0 0 5 )。结论 :SLE患者体内存在T细胞亚群异常活化 ;活动期SLE患者T淋巴细胞亚群DNA 1表达增高 ;SLE患者CD+8细胞DNAM 1表达异常与SLEDAI、抗dsDNA抗体、C3和C4 补体之间有明显相关 ,CD+8细胞活化程度可能与SLE疾病严重程度有关 ,故DNAM 1可能参与了SLE的免疫发病机理。

Objective:To investigate functional of DNAM 1 antigen in T cell subsets activation process from patients with SLE and determine whether they are correlated with the pathogenesis of SLE.Methods:The peripheral blood monouclear cells from 31 SLE and 30 healthy volunteers were stimulated with PHA.Those cells were quantified using flow cytometric analysis with three colour immunofluorescent staining method.The levels of anti dsDNA,complement C 3 and C 4 were measured as well as.Results:The percentages of DNAM 1 positive from SLE on CD + 4 and CD + 8 T cells were increased,compared with that of the controls (P<0.01).The expression of DNAM 1 on CD + 4 and CD + 8 T cells from active SLE were all significantly higher than that of controls and inactive SLE(P<0.01).However,there was no difference between that from inactive SLE and that from the controls(P>0.05).The DNAM 1 expression on CD + 8 cells from SLE showed relatively high positive correlation with SLEDAI and anti dsDNA antibody and that on CD + 8 T cells have negative correlation with C 3 and C 4 complement(P<0.05).However,that on CD + 4 have no correlation with SLEDAI?anti dsDNA antibody?C 3 and C 4 complement(P>0.05).Conclusion:There are some abnormal activated T cell subsets in SLE.The expression of DNAM 1 on CD + 4?CD + 8 T cells with active SLE are increased.That on CD + 8 cells are correlated to SLEDAI?anti dsDNA antibody?C 3 and C 4 complement and there is a clinical correlation with it's levels.So DNAM 1 may play an important roles in the mechanisms of immune activation and immunopathology in SLE.