反相高效液相色谱法测定复方谷氨酰胺中呱仑酸钠的含量

Determination of sodium gualenate in compound glutamine by RP-HPLC

目的 :建立复方谷氨酰胺中呱仑酸钠的反相高效液相色谱测定方法。方法 :色谱柱InertsilCN 3(5μm ,4.6mm× 2 50mm) ,磷酸盐缓冲液 (pH 7) 乙腈 (50∶50 )为流动相 ,检测波长 2 90nm ,流速1 .0mL·min- 1 。结果 :呱仑酸钠的浓度在 1 .0～ 6 .0mg·L- 1 范围内呈线形关系 ,平均回收率为 99.8% ,RSD为 0 .4 % (n =8)。结论 :本法具方法简便 ,结果准确 。

OBJECTIVE To establish an RP HPLC method for determination of sodium gualenate in compound glutamine.METHODS The analysis was carried out using Inertsil CN 3 column (5 μm, 4.6 mm×250 mm). The mobile phase consisted of phosphate buffer (pH=7) acetonitrile (50∶50), detection was set at 290 nm, the flow rate was 1.0 mL·min -1 .RESULTS The linear range of sodium gualenate was 1.0 ～ 6.0 mg·L -1 , the average recovery was 99.8% with RSD of 0.4% ( n =8).CONCLUSIONS This method provides a simple and feasible way to control the quality of compound glutamin.