血管内皮生长因子与转化生长因子β在非小细胞肺癌的表达及其相关性

Expression of and correlation between the vascular endothelial growth factor and transforming growth factor beta in patients with non-small cell lung cancer

目的 研究血管内皮生长因子 (VEGF)与转化生长因子 β(TGFβ)在非小细胞肺癌的表达及其相关性。方法 应用SP免疫组化方法研究VEGF和TGFβ1在 5 0例非小细胞肺癌 (NSCLC)的表达。结果 VEGF阳性显色主要分布于癌细胞的胞浆内 ,表达率为 66% ,其表达与NSCLC的病期进展、淋巴结转移有关 ;TGFβ1阳性表达主要分布于癌细胞的胞浆内 ,细胞核内无表达 ,表达率为 60 % ,其表达与NSCLC的病期进展、淋巴结转移有关 ,且两者表达在癌组织与癌旁组织之间均有显著性统计学意义。 3 3例VEGF阳性表达标本中 ,单纯VEGF表达阳性者 ( 9/3 3 )明显低于VEGF与TGFβ1同时表达阳性者 ( 2 4/3 3 ) ;3 0例TGFβ1表达阳性者中 ,单纯TGFβ1表达阳性 ( 6/3 0 )也明显低于二者均阳性表达者 ( 2 4/3 0 ) ,VEGF与TGFβ1表达之间存在正相关关系 (r =0 .3 62 ,P <0 .0 1)。结论 VEGF和TGFβ1在NSCLC均有较好的表达。

Objective To investigate the expression of vascular endothelial growth factor (VEGF) and transforming growth factor beta (TGFβ) and the correlation between them in patients with non-small cell lung cancer.Methods We examined the expression of VEGF protein and TGFβ 1 protein in surgical specimens of 50 patients with NSCLC by SP immunohistochemistry stain method. Results VEGF-positive rate was 66.00%, and VEGF was stained mainly in the cytoplasm of tumor cells of NSCLC. The positive expression rate of VEGF in stage Ⅱ and that in stage Ⅲ were significantly higher than that in stage Ⅰ (P<0.05). Compared with the patients without lymph node metastasis, the positive expression of VEGF was significantly higher in the patients with lymph node metastasis (P<0.05). No significant association was found between the VEGF expression and the histological types (P>0.05). Similar to VEGF expression, positive expression rate of TGFβ 1 in stage Ⅱ and stage Ⅲ were significantly higher than that in stage Ⅰ (P<0.05), and the positive expression of TGFβ 1 was significantly higher in the patients with lymph node metastasis than that without lymph node metastasis(P<0.05). No significant association was found between the TGFβ 1 expression and the characteristics of the histological types (P>0.05) of patients in NSCLC. The significant differences of positive expressions of VEGF and TGFβ 1 between carcinomatous and para-carcinomatous specimens were found. Of the 33 specimens with VEGF expression, the case of single VEGF expression (9/33) was evidently less than that of simultaneous expression of VEGF and TGFβ 1 (24/33). The same finding presented in case of the single TGFβ 1 expression (6/30) compared with that of the simultaneous expression of TGFβ 1 and VEGF (24/30). A positive correlation of VEGF expression and TGFβ 1 expression was found (r=0.362, P<0.01). Conclusions The cooperation of VEGF and TGFβ 1 highly promotes the progression and lymph node metastasis of NSCLC partly because of the positive regulation of TGFβ 1 to VEGF. Further study on expression characteristics and regulating mechanisms of VEGF and TGFβ 1 may provide the theoretical basis for anti-tumor gene therapy in NSCLC.